Simple, accurate, precise, and sensitive ratio spectra derivative spectrophotometric method for simultaneous estimation of Pantoprazole (PANTO) and Levosulpiride (LEVO) in Pharmaceutical dosage form have been developed and validated. The ratio derivative spectroscopic method involves measurement of first derivative amplitude of ratio spectra at 276.8 nm for PANTO and 241.4 nm for LEVO as two wavelengths for estimation. Beer's law is obeyed in the concentration range of 2-10 μg/ml for both PANTO and LEVO. LOD and LOQ values for PANTO were found to be 0.34 and 1.02 μg/ml and at 276.8 nm, respectively. LOD and LOQ values for LEVO were found to be 0.60 and 1.81 μg/ml and at 241.4 nm, respectively. The method was validated statistically and recovery studies were carried out. It was found to be accurate, precise and reproducible. The method was applied to the assay of the drugs in Tablet dosage form, which were found in the range of 99.72±0.31 for Pantoprazole and 99.94±0.18 for Levosulpiride of the labeled value for both Pantoprazole and Levosulpiride. Hence, the method herein described can be successfully applied in quality control of synthetic mixture.
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